Liquid Handling Boot Camp:Resource Page
Resource page for the "Liquid Handling Boot Camp" short course
To be taught at the SLAS 2012 Annual Conference, February 2012
This short course is designed to assist those who use liquid handling robots in the biopharmaceutical, and other life science fields. It teaches students to recognize and understand the variables associated with gaining maximum performance out of their liquid handlers.
The class will begin with a concise review of the science and theory of automated liquid handling. Students will then have the opportunity to apply this knowledge in hands-on exercises with a variety of robotic liquid handlers using various liquids, seeing immediate liquid delivery performance data, and experimenting with different techniques.
- Introduction to liquid handling robots - types of laboratory automation and technology background
- Safety issues - common safety risks and gross performance issues
- Liquid handling terminology, issues and techniques - definitions of terms, failure modes and basic techniques
- Reagent and chemical compatibility and handling issues - aggressive solvents, high viscosity liquids and "foaming"
- Validation and QC techniques and options - definitions, why, when, suggestions and regulatory issues
- Decontamination protocols - why, and recommended agents
- Hands-on pipetting - 2/3 of total class time
- on real robots
- aqueous liquids of varying viscosities
Hands-on Lab Exercises
During the course, attendees have the opportunity to apply what they have learned on a number of actual liquid handling robots. The instructors are there to assist.
- Dispense Speed (non-contact dispense)
- Dispense Speed (contact dispense)
- Dispense Speed for High Viscosity fluids
- Aspirate Speed for High Viscosity fluids
- Air Gaps for High Viscosity fluids
- Mixing – varying number of mixes
- if time, class suggested experiments
University of California, San Diego
San Diego, California, USA
Thousand Oaks, California, USA
- “Air-Displacement and Positive-Displacement Pipetting Techniques”, Gilson, Inc., website, file pdfid240.pdf
- “Microarrays Methods and Applications”, Chapter 2: Laboratory Automation for Microarray Experimentation, Hardiman, Gary, DNA Press, 2003
- “Marks’ Standard Handbook for Mechanical Engineers”, Avallone & Baumeister, McGraw-Hill Professional, 10th Edition, 1996
- “Qualification of robotic laboratory equipment”, Caillet C, Pegon Y, Le Neel T, Morin D, Baudiment C, Truchaud A, Journal of the Association for Laboratory Automation - February 2005 (Vol. 10, Issue 1, Pages 48-53)
- “Comparison of Absorbance and Fluorescence Methods for Determining Liquid Dispensing Precision”, Petersen J, Nguyen J, Journal of the Association for Laboratory Automation - April 2005 (Vol. 10, Issue 2, Pages 82-87)
- “Critical Evaluation of the Mosquito (A Low Volume Liquid Handler): A Tool for Drug Discovery”, Mosser S, LabAutomation2006, Track 2, 11:30am, Palm Springs, CA, 2006
Equipment we are working on for the Experiments - with a special thanks to the Vendors providing the equipment.
Beckman Biomek® NXp
Artel MVS® systems for the performance reads
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