Maren Plüger1, Michael Katzlinger2, Anita Eigner1, Jiri Kopecky3, Maria Hirschler1, Linda Kotnik1, Katrin Fuchslueger1, Julia Schweiger1, Christoph Wiesner1, Andreas Eger1, Yvonne Fitzgerald2, Cathy Olsen2, Wolfgang Schütt1, and Harald Hundsberger1
1Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, Piaristengassse 1, Krems, Austria; 2Molecular Devices, Inc., 1311 Orleans Drive, Sunnyvale, CA 94089; 3Czech Academy of Sciences, Trebon, Czech Republic
Inflammation is accompanied by increased endothelial chemokine production and adhesion molecule expression, which may result in an extensive neutrophil infiltration. As such, the search for novel anti-inflammatory substances able to downregulate these parameters, as well as the tissue damage, holds therapeutic promise. Here we describe how Alpha Technology, a bead-based, homogeneous assay for studying molecular interactions in a microplate format, has been used to detect anti-inflammatory metabolites from cyanobacteria in human endothelial cell-based in vitro assays. We show the quantification of cytokines (TNF-alpha) down to picogram levels with Molecular Devices' SpectraMax® Paradigm® Modular Multi-Mode Reader. Alpha Technology can be performed on the SpectraMax Paradigm Reader in various plate formats ranging from 96-well to 1536-well.
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