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The PrestoBlue™ Cell Viability Reagent:
Reliable Cell Viability Data in as Few as 10 Minutes

Bonnie J. Hanson, Michael Hancock, Uma Lakshmipathy, Lesiha Kopp
Life Technologies, 501 Charmany Dr., Madison, WI 53719

PrestoBlue™ Cell Viability Reagent is a resazurin-based solution that uses the reducing ability of living cells to quantitatively measure cell proliferation. When cells are alive and healthy, they maintain a reducing environment within their cytosol. Upon entering a living cell, PrestoBlue™ Reagent is reduced to resorufin which is red in color and highly fluorescent. The health of the cell can be monitored by the change in fluorescence and/or absorbance. Metabolically active cells continuously convert the PrestoBlue™ Reagent. Non-viable cells cannot reduce the indicator dye and therefore do not generate a change in signal.

Whereas other commercially available resazurin solutions (such as CellTiter-Blue® or alamarBlue® reagents) require a 1-4 hour incubation in order to generate a significant signal, we show that PrestoBlue™ reagent generates a linear signal with excellent sensitivity in response to cell number in just 10 minutes. We also show that the reagent can be used with a wide variety of mammalian and non-mammalian cell types including primary and stem cells, insect cells, and bacterial cells. In addition, we show that the IC50 values generated from cytotoxic agents is similar for PrestoBlue™ reagent as other common cell viability reagents like CellTiter-Glo® and MTT.

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