Turbidometric performance of Thermo Scientific Multiskan GO
Jorma Lampinen, Hanna Granö-Fabritius, Marika Raitio, Tom Pessala and Reija-Riitta Harinen, Thermo Fisher Scientific, Sample Preparation & Analysis, Vantaa, Finland
Turbidometry is a special type of photometric measurements where one measures the scattering of the light caused by solid particles in the solution. Turbidometry can be applied in a variety of research areas, for example water and beverage quality control and microbial growth. It is based on the fact that all particles, including microbes, are capable of light scattering. The amount of light scattered is directly proportional to the amount of particles present in the liquid when the shape and size of the particles remains the same. Turbidity is commonly measured using a special turbidometer/nephelometer devices, but it can also be determined using a normal microplate photometer or spectrophotometer.
Formazine solutions are traditionally used to verify and calibrate turbidometric devices because of their high reproducibility. First part of this work describes the verification of the turbidometric performance of Thermo Scientific Multiskan GO with a standard formazine solution. The second part of the work shows an example of how to measure the bacterial growth and antimicrobial growth inhibition using turbidometric measurement with a microplate spectrophotometer.
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