Modified Carbon Cloth Electrode with Applications in Neurochemical Analysis

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Modifed Carbon Cloth Electrode Array with Applications in Neurochemical Analysis

 Susan Warren*1,Dhanraj Rathod2, Timothy McCormac3 and Eithne Dempsey1

1Centre for Research in Electroanalytical Technologies (CREATE), Centre of Applied Science for Health (CASH), Dept. Science, ITT Dublin, Tallaght Dublin 24.�2Centre for Research in Electroanalytical Technologies (CREATE), Dept. Science, �ITT Dublin, Tallaght Dublin 24, Ireland.

�3Electrochemistry Research Group, Dundalk Institute of Technology, Dundalk, Co. Louth, Ireland.� �

This work is concerned with the electrochemical monitoring of key neurochemicals such as dopamine, glutamate, glucose and lactate. The proposed system is based upon the use of a nanoparticle decorated micro mesh carbon array with selectivity for each target analyte. The modified microelectrodes are integrated within a flowcell connected to a microdialysis sampling probe, with potential for in vitro/in vivo monitoring. Lactate and glucose play a key role in the metabolism and energetics of the brain, and glucose levels are depleted during ischemic episodes. Dopamine is an important neurotransmitter involved in both motor and cognitive functions (nM range) and is electroactive. Two approaches to the catalytic (bio)sensing system will be presented (a) the controlled electrodeposition of metallic nanoparticles (Pt, Au, Pd) on carbon fibre micromesh electrodes for direct non-enzymatic glucose and dopamine oxidation (b) the covalent attachment of lactate oxidase onto Pt nanoparticle decorated carbon surfaces, enabling hydrogen peroxide detection while providing a stable environment for the biomolecule.