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MP55:ANALYZING METHYLATED ARGININES USING CAPILLARY ELECTROPHORESIS AND LASER INDUCED FLUORESCENCE DETECTION

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Christa Snyder,3 Thomas Linz,1,2 and Susan M. Lunte1,2
1 Department of Chemistry, 2 Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS 66045
3 Wittenberg University, Springfield, OH 45501

The methylated arginines monomethylarginine (MMA), asymmetrical dimethylarginine (ADMA) and symmetrical dimethylarginine (SDMA) have been shown to be independent predictors of cardiovascular disease (CVD). As a result, an analytical method was developed to determine the concentrations of MAs in human plasma in order to track the onset and progression of CVD. Capillary electrophoresis (CE) with laser induced fluorescence detection (LIF) was used for the analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-derivatized MAs. The optimization of run buffer composition, focusing on sodium borate and sulfylbutylether b-cyclodextrin (SBE-b-CD) concentrations, was crucial in obtaining adequate resolution between the analytes of interest. Under optimal conditions, SDMA, ADMA, MMA and arginine were baseline resolved and the limits of detection for those species were 41 amol, 30 amol, 41 amol and 10 amol, respectively. Plasma analysis was performed using strong cation exchange solid phase extraction (SPE) tips to preconcentrate the analytes of interest prior to derivatization. Recovery of MAs from the SPE tips was determined to be 43%.


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