MP29: Electrophysiological Sorting of Pluripotent Stem Cell-DerivedCardiomyocytes in a Microfluidic Platform

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Frank B. Myers1, Oscar J. Abilez2, Chris K. Zarins2,3, Luke P. Lee1

1Department of Bioengineering, University of California, Berkeley, USA
2Department of Bioengineering,
3Department of Surgery, Stanford University, USA

The heterogeneity of stem cell-derived populations is a major translational bottleneck for regenerative medicine. Conventional strategies for cell puri!cation (FACS, genetic selection) are not suitable for clinical application. Interestingly, many stem cell therapies, including heart therapies, involve the use of electrically-excitable cells, and the electrophysiological signals these cells generate can be used to non-invasively identify them with high specificity. We are developing a radically new approach to cell sorting which distinguishes cardiomyocytes and other cells based on their electrophysiology. The system electrically stimulates individual cells as they flow through a microchannel, and observes their electrical response.

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