Paige N. Feikert1, Krishna Vattipalli1, 2, and Shalini Prasad1, 2
1Bioengineering Program, Wichita State University, Wichita, KS.
2Bioengineering Program and Department of Electrical Engineering and Computer Science, Wichita State University, Wichita, KS.
The goal of this work is to understand the role of nano confinement in designing biosensors. We have been investigating silicon based micro devices incorporated with nanoporous membranes in designing sensors. We have observed that nanoporous membranes enable nanoscale size based confinement of biomolecules such as proteins onto micro platforms. This in turn enhances the concentration of the biomolecules which in turn enhances the sensitivity in detecting biomolecules. It is critical that ultralow detection of biomolecules be achieved as they have significant impact in designing diagnostics platforms for early disease diagnosis. Commercially available nano-porous membranes made out of anodized alumina and polycarbonate are evaluated for their role in nano-confinement and enhancing sensitivity of detection. In this biosensor configuration sandwich assay, an electrical double layer is formed between a test protein (C-reactive protein) and the gold surface underneath the porous membrane. Using electrical impedance spectroscopy, the capacitance changes in the electrical double layer, translating to the sensitivity and the linear dose response over a large dynamic range will be analyzed for each of the physical characteristic of the porous membrane – pore densities, height of the pore and diameter of pore.
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