MP03:microfluidic fraction collection

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Christopher A. Baker and Michael G. Roper Department of Chemistry and Biochemistry, Florida State University, Tallahassee Florida 32306


A microfluidic device for fraction collection following electrophoretic separation is described. Multiphysics simulations were used to evaluate operating principles and optimize device geometry. Separation and fraction collection were demonstrated using a fluorescently-labeled amino acid mixture. Multiphysics simulations demonstrated a chip based pneumatic flow switching technique suitable for high flow rate (mL/min) applications such as fraction collection following HPLC separations. Software for automated peak detection and characterization is also described.

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