M145:DeLaura:Human-based Systems for in vitro Modeling: The Development and Characterization of Induced Pluripotent Stem Cell (iPSC)-derived Neurons for Preclinical Use
Cellular Dynamics International
Central nervous system (CNS) disorders affect millions of people worldwide. With the complexity of the human brain, it is difficult to model. Currently, simplified aspects of its function are modeled using primary cells harvested from cadavers, embryonic stem cell-derived neural progenitor cells, immortalized cell lines, and animal models during drug discovery research. However, issues of biological relevance, reproducibility, and scalability can arise, and the reliance on inferior models may result in drug-induced neurotoxicity not being observed until late-stage clinical trials or after the introduction of a drug to the marketplace. A significant push within the drug discovery field has been the development and validation of a diverse array of more relevant biological systems, including those derived from stem cell culture systems. We have developed human neurons derived from induced pluripotent stem cells (iPSCs) that offer the potential to overcome these limitations. These iPSC-derived neurons are >95% pure and exhibit characteristic neuronal morphology, gene and protein expression, and electrophysiological properties. Data highlighting their generation, characterization, and expression of native behavior will be presented. The development of human iPSC-derived neurons that recapitulate in vivo neuronal function will enable powerful new strategies for drug discovery, neurotoxicity testing, and disease research.
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