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Category:NIH Chemical Genomics Center Assay Guidance Manual

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The NIH - AGM is a manual is intended to provide guidance in the area of biological assay development, screening and compound evaluation. In this regard an assay is defined by a set of reagents that produce a detectable signal allowing a biological process to be quantified. In general, the quality of an assay is defined by the robustness and reproducibility of this signal in the absence of any test compounds or in the presence of inactive compounds. This robustness will depend on the type of signal measured (absorbance, fluorescence, radioactivity etc), reagents, reaction conditions and analytical and automation instrumentation employed. The quality of the HTS is then defined by the behavior of this assay system when screened against a collection of compounds. These two general concepts, assay quality and screen quality, are discussed with specific examples in the chapters of this manual.


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Pages in category "NIH Chemical Genomics Center Assay Guidance Manual"

The following 4 pages are in this category, out of 4 total.